Separation: The mobile phase interacts Along with the stationary period during the column as well as the analytes inside the sample. This conversation impacts how swiftly Every analyte travels through the column, leading to their separation.
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
物質の電気化学的な性質を利用した検出器。pHの変動や酸化還元電位の変動を用いて測定を行う。
In reversed-phase HPLC the purchase of elution is the alternative that in a normal-section separation, with more polar solutes eluting 1st. Increasing the polarity of your mobile stage results in extended retention situations. Shorter retention times demand a mobile period of lessen polarity.
It seems odd the more widespread method of liquid chromatography is identified as reverse-period instead of ordinary stage. You might remember that on the list of earliest examples of chromatography was Mikhail Tswett’s separation of plant pigments employing a polar column of calcium carbonate along with a nonpolar mobile phase of petroleum ether. The assignment of usual and reversed, hence, is all about precedence.
규제 약물(마약, 합성 마약, 대마, 각성제, 향정신성 의약품, 아편양제제 등), 반도핑 관련(금지 물질, 금지 get more info 약물, 스테로이드 등), 약물 대사물
The pump is the guts of the HPLC system. It provides the mobile section at a continuing and high strain (around four hundred atm) from the column. Steady movement rate is significant for achieving best separation and preserving reproducibility. Components to consider when deciding upon a circulation rate involve:
This change in interaction times brings about the separation of analytes as they exit the column at diverse times.
A pump forces a solvent via a column under high pressures of as much as 400 atmospheres. The column packing content or adsorbent or stationary stage is often a granular materials of good particles which include silica or polymers.
*본 포스팅의 저작권은 써모 피셔 사이언티픽에 있으며, 콘텐츠의 무단 복제 및 수정, 재배포를 금지합니다.
The pressurized liquid is typically a mixture of solvents such as drinking water, acetonitrile and/or methanol and it is called the cellular period.
Stream rate: Circulation amount adjustment has an effect on how quickly analytes move with the column. An ideal flow charge balances separation efficiency with Investigation time.
The injector introduces read more a exact volume on the sample solution in the cellular stage stream. Many injection approaches exist, with loop injection currently being a common method.